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KMID : 0380620130450010001
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Korean Journal of Food Science and Technology
2013 Volume.45 No. 1 p.1 ~ p.7
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Development of Detection Method for Niphon spinosus, Epinephelus bruneus, and Epinephelus septemfasciatus using 16S rRNA Gene
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Park Yong-Chjun
Jung Yong-Hyun
Kim Mi-Ra
Shin Joon-Ho
Kim Kyu-Heon
Lee Jae-Hwang
Cho Tae-Yong
Lee Hwa-Jung
Lee Sang-Jae
Han Sang-Bae
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Abstract
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Abstract Niphon spinosus, Epinephelus bruneus, and Epinephelus septemfasciatus are involved in the Perciformes Order and Serranidae Family. When E. bruneus and E. septemfasciatus are fully grown, the striped pattern on the body gradually disappears. Therefore, morphological classification of adult fishes is quite difficult to identify the differences to N. spinosus. In this study, we investigate the method to differentiate those using PCR. To design the primers, 16S rRNA region of N. spinosus, E. bruneus, and E. septemfasciatus registered in the GeneBank (www.ncbi.nlm.nih.gov) have been used and for the analysis, Bio Edit ver. 7.0.9.0 was used. As a result, it was design NS-003-F/NS-005-R (136 bp), EB-001-F/EB-002- R (181 bp), and ES-001-F/ES-001-R (123 bp) primers for the differentiation of each 3 different fishes. Therefore, the species-specific primer sets would be a useful tool for scientific and speedy differentiation against the illegal distribution for consumer protection.
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KEYWORD
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Niphon spinosus, Epinephelus bruneus, Epinephelus septemfasciatus, PCR (Polymerase Chain Reaction), speciesspecific primer, 16S rRNA
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